Effects of <i>TOX3 Regulation using CRISPR-Cas9 genome editing and the corresponding effects on susceptibility to Breast Cancer

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Ashy, Cody C
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One in eight women will be diagnosed with breast cancer. Due to the prevalence, high morbidity, and high costs of treatment, new screening and prevention methods need to be developed. TOX3 downregulation is correlated to increased breast cancer risk. This study investigated the TOX3 locus, utilizing state of the art CRISPR/Cas9 technology to reduce gene expression in rats and develop a model of susceptibility. In addition, TOX3 overexpression was achieved using a Cas9 mutant (dCas9-VP64) that is not able to cut the gene. It has been previously established in many known oncogenes that upregulation transformed healthy cells to a cancerous state. Using this technology, we theoretically could transform normal mammary epithelial cell line, MCF10a, cells into a cancerous state. This invention can serve as an efficient and inexpensive screening tool to study candidate genes and determine new oncogenes.
<i>TOX3, CRISPR-cas9, Breast Cancer, susceptibility