Temporal Expression of Importins IPO4, IPO11, IPO13, and KPNB1 in <i>Lytechinus variegatus</i> During Embryogenesis
| dc.contributor.advisor | Byrum, Christine A | |
| dc.contributor.author | Baker, Ross | |
| dc.date.accessioned | 2022-03-25T17:32:40Z | |
| dc.date.available | 2022-03-25T17:32:40Z | |
| dc.date.created | 2014-05 | |
| dc.date.submitted | May 2014 | |
| dc.description.abstract | Temporal expression of the importins IPO4, IPO11, IPO13 and KPNB1 was examined in <i>Lytechinus variegatus</i> during embryogenesis to determine whether these genes are differentially expressed or ubiquitously distributed during development. Utilizing template cDNA isolated from <i>L. variegatus</i> embryos at 0, 3, 6, 12, 18, 24, and 30 hours post fertilization (hpf), reverse transcriptase PCR (RT-PCR) was performed to amplify the target genes. In addition, transcriptome and domain analyses were performed on each of the karyopherins to identify expression patterns in a related sea urchin <i>Strongylocentrotus purpuratus</i> (the purple sea urchin) from the Pacific coast and to determine the degree of structural similarity present in the importin sequences of both species. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://repository.library.cofc.edu/handle/123456789/4914 | |
| dc.language.iso | en_US | |
| dc.subject | Karyopherin | |
| dc.subject | Importin | |
| dc.subject | <i>Lytechinus variegatus<i/> | |
| dc.subject | IPO4 | |
| dc.subject | IPO11 | |
| dc.subject | IPO13 | |
| dc.subject | KPNB1 | |
| dc.title | Temporal Expression of Importins IPO4, IPO11, IPO13, and KPNB1 in <i>Lytechinus variegatus</i> During Embryogenesis | |
| dc.type.genre | thesis | |
| dc.type.material | text | |
| thesis.degree.department | Biology | |
| thesis.degree.discipline | Biology | |
| thesis.degree.grantor | College of Charleston | |
| thesis.degree.name | Bachelor of Science |