Emerging contaminants in the marine environment: An in vitro study of the immunotoxicological effects of DE-71, a commercial polybrominated diphenylether mixture, and perfluorooctane sulfonate on dolphin and murine immune cells

dc.contributor.advisorFair, Patricia Aen_US
dc.contributor.authorWirth, Jenaen_US
dc.contributor.sponsorMarine Biologyen_US
dc.date.accepted01/01/2012en_US
dc.date.accessioned2016-10-18T16:14:26Z
dc.date.available2016-10-18T16:14:26Z
dc.date.completed2012en_US
dc.date.issued2013-03-08
dc.descriptionThesis (M.S.) College of Charleston, South Carolina-The Graduate School, 2012en_US
dc.descriptionCommittee members: Patricia A Fair, Margie M Peden-Adams, Allan Strand, David Owens, Natasha White, Amy T McCandlessen_US
dc.descriptiondolphin, immunotoxicology, mouse, PBDE, PFOSen_US
dc.description.abstractThe effects of emerging contaminants on the immune system of mammals are poorly understood, with limited information available about the immunotoxicological effects of perfluorooctane sulfonate (PFOS) and DE-71, a polybrominated diphenyl ether (PBDE) mixture, at environmentally relevant exposures. Due to challenges of working with dolphins, this study assessed the effects of in vitro exposures of PFOS and DE-71 on immune functions in dolphins and mice to determine the predictability of in vitro effects to in vivo observations as part of a parallelogram approach to assessing risk. Bottlenose dolphin peripheral blood leukocytes and B6C3F1 mouse splenocytes were exposed in culture to PFOS at 0, 0.01, 0.05, 0.1, 0.5, 1.0 or 5.0 μg/mL concentrations or DE-71 at 0, 0.025, 0.05, 0.25, 0.5, 2.5, 5.0, 25 or 50 μg/mL concentrations. The immune endpoints assessed were natural killer (NK) cell activity and lymphocyte proliferation. PFOS significantly decreased NK cell activity at 0.01, 0.05, 0.1, 0.5, and 1.0 μg/mL concentrations in mouse splenocytes, but did not significantly alter dolphin NK cell activity. Mitogen-induced T cell proliferation was significantly decreased in murine cells beginning at the 0.05 μg/mL treatment, but not in dolphin cells. B cell proliferation was not statistically altered by PFOS exposure in either dolphins or mice. DE-71 in vitro exposure did not significantly alter NK cell activity or lymphocyte proliferation in dolphins. It also did not alter lymphocyte proliferation in mice, but did augment NK cell activity beginning with the 0.05 μg/mL treatment. Using these in vitro models and previous studies on in vivo models, the parallelogram approach was able to estimate potential effects of PBDE and PFOS in free-ranging bottlenose dolphin immune function.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3171
dc.languageenen_US
dc.subjectToxicologyen_US
dc.subjectImmunologyen_US
dc.titleEmerging contaminants in the marine environment: An in vitro study of the immunotoxicological effects of DE-71, a commercial polybrominated diphenylether mixture, and perfluorooctane sulfonate on dolphin and murine immune cellsen_US
dc.typeThesisen_US
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