Browsing by Subject "<i>Lytechinus variegatus<i/>"

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    Temporal Expression of Importins IPO4, IPO11, IPO13, and KPNB1 in <i>Lytechinus variegatus</i> During Embryogenesis
    Baker, Ross; Byrum, Christine A
    Temporal expression of the importins IPO4, IPO11, IPO13 and KPNB1 was examined in <i>Lytechinus variegatus</i> during embryogenesis to determine whether these genes are differentially expressed or ubiquitously distributed during development. Utilizing template cDNA isolated from <i>L. variegatus</i> embryos at 0, 3, 6, 12, 18, 24, and 30 hours post fertilization (hpf), reverse transcriptase PCR (RT-PCR) was performed to amplify the target genes. In addition, transcriptome and domain analyses were performed on each of the karyopherins to identify expression patterns in a related sea urchin <i>Strongylocentrotus purpuratus</i> (the purple sea urchin) from the Pacific coast and to determine the degree of structural similarity present in the importin sequences of both species.